Cell sorting is an aerosol-generating procedure, with greatly increases the risk of airway transmission of hazardous and infectious material. Aerosol incidents are not uncommon during normal sort procedures. These incidents can be caused by sample clogging and other disturbances that increase physical instability of the fragile single-cell stream.
Updated 9/18/17
Tissue microarray (TMA) for breast cancer.
File: Breast_TMA_Information.pdfThe tip sheet is a reference tool based on wiscweb's beginner tip sheet for learning to use WordPress. The tip sheet includes instructions on how to:
1. Access WordPress
2. Make a new page
3. Edit existing pages
4. Create posts
5. Use menus
7. Use the Media Library
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Fill out the case submission form for your submitted patient. All information is needed to present your patient's case to the Precision Medicine Molecular Tumor Board.
File: Case-Submission-Form.docxThe cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and measuring its intensity. The dye stains DNA stoichiometrically, allowing differentiations of cells in G0/G1, S phase, and G2/M, as well as identification of aneuploid populations. A variety of staining protocols can be adapted for different sample types, but the general analysis remains the same.
Updated 9/18/17
https://wwwtest.cancer.wisc.edu/research/wp-admin/post-new.php?post_type=uw_documents#
File: Clinical-Research-Collaboration-SOP.pdfTissue Microarray (TMA) for colorectal and anal cancer.
File: colorectal-anal-cancer-TMA-information.pdfCompensation beads offer a variety of benefits. The most obvious of these is that they allow you to save more of your cells for your experimental conditions rather than controls. Additionally, compensation beads stain more brightly and uniformly than cells, allowing for easy compensation of colors that may be only on rare events in the cells sample. Importantly, compensation beads can be used with the same antibodies you use for your experiment ensuring a perfect fluorochrome match.
Updated 9/18/17
CPOD Proposal for Service
File: proposal_for_service.pdfYang et al.
Multifunctional and water-soluble superparamagnetic iron oxide (SPIO) nanocarriers were developed for targeted drug delivery and positron emission tomography/magnetic resonance imaging (PET/MRI) dualmodality
imaging of tumors with integrin avb3 expression. An anticancer drug was conjugated onto the PEGylated SPIO nanocarriers via pH-sensitive bonds. Tumor-targeting ligands, cyclo(Arg-Gly-Asp-D-Phe-Cys) (c(RGDfC)) peptides, and PET 64Cu chelators, macrocyclic 1,4,7-triazacyclononane-N, N0, N00-triacetic acid (NOTA), were conjugated onto the distal ends of the PEG arms. The effectiveness of the SPIO nanocarriers as an MRI contrast agent was evaluated via an in vitro r2 MRI relaxivity measurement. cRGD-conjugated SPIO nanocarriers exhibited a higher level of cellular uptake than cRGD-free ones in vitro. Moreover, cRGD-conjugated SPIO nanocarriers showed a much higher level of tumor accumulation than cRGD-free ones according to non-invasive and quantitative PET imaging, and ex vivo biodistribution studies. Thus, these SPIO nanocarriers demonstrated promising properties for combined targeted anticancer drug delivery and PET/MRI dual-modality imaging of tumors.