{"id":12058,"date":"2019-08-14T10:32:15","date_gmt":"2019-08-14T15:32:15","guid":{"rendered":"https:\/\/cancer.wisc.edu\/research\/?p=12058"},"modified":"2019-08-14T10:56:40","modified_gmt":"2019-08-14T15:56:40","slug":"on-rigor-reproducibility-cell-line-authentication","status":"publish","type":"post","link":"https:\/\/wwwtest.cancer.wisc.edu\/research\/on-rigor-reproducibility-cell-line-authentication\/","title":{"rendered":"On Rigor &#038; Reproducibility: Cell Line Authentication"},"content":{"rendered":"<p>It has been estimated that 18-36% of cell lines utilized in biomedical research are contaminated and\/or completely misidentified. Verification of human and stem cell line identity is critical to the validity of the scientific data.<\/p>\n<p><a href=\"https:\/\/research.pathology.wisc.edu\/trip-home\/\">TRIPath<\/a> offers Short Tandem Repeat (STR) analysis to verify human and stem cell line authenticity. Read more on <a href=\"https:\/\/research.pathology.wisc.edu\/cell-line-authentication\/\">Cell Line Authentication<\/a>, and plan to attend the <a href=\"https:\/\/www.promega.com\/c\/trip-cla-seminar-registration\/\">upcoming Lunch Seminar on Aug. 21st.<\/a><\/p>\n<table>\n<tbody>\n<tr>\n<td>\n<p><figure id=\"attachment_12063\" class=\"wp-caption aligncenter\" style=\"max-width: 300px;\" aria-label=\"Photo courtesy of Karla Esbona, 2019\"><img decoding=\"async\" loading=\"lazy\" class=\"size-medium wp-image-12063\" src=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication-300x185.png\" alt=\"Vials held up below droppers in the cell line authentication process.\" width=\"300\" height=\"185\" srcset=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication-300x185.png 300w, https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication.png 600w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><figcaption class=\"wp-caption-text\">Photo courtesy of Karla Esbona, 2019<\/figcaption><\/figure><\/td>\n<td>\n<p><figure id=\"attachment_12062\" class=\"wp-caption aligncenter\" style=\"max-width: 300px;\" aria-label=\"Human Cell Line Authentication: Analysis of 15 Microsatellite (STR) Loci and Amelogenin (Gender Specific Loci)\"><img decoding=\"async\" loading=\"lazy\" class=\"size-medium wp-image-12062\" src=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication-2-300x107.png\" alt=\"Screenshot of chart with the header &quot;Human Cell Line Authentication: Analysis of 15 Microsatellite (STR) Loci and Amelogenin (Gender Specific Loci)&quot;\" width=\"300\" height=\"107\" srcset=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication-2-300x107.png 300w, https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-content\/uploads\/2019\/08\/Cell-Authentication-2.png 600w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><figcaption class=\"wp-caption-text\">Human Cell Line Authentication: Analysis of 15 Microsatellite (STR) Loci and Amelogenin (Gender Specific Loci)<\/figcaption><\/figure><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><em>This article was originally published in the BadgerConnector newsletter. To stay up to date with UWCCC shared resources,\u00a0<a href=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/shared-resources\/badgerconnector\/\">subscribe to the newsletter online<\/a>.<\/em><\/p>\n<p><strong>Past articles from our Rigor &amp; Reproducibility series:<\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/wwwtest.cancer.wisc.edu\/research\/how-research-cores-help-with-rigor-reproducibility\/\">How Research Cores Help with Rigor &amp; Reproducibility<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>It has been estimated that 18-36% of cell lines utilized in biomedical research are contaminated and\/or completely misidentified. Verification of human and stem cell line identity is critical to the validity of the scientific data. &hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0},"categories":[22],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/12058"}],"collection":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/comments?post=12058"}],"version-history":[{"count":5,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/12058\/revisions"}],"predecessor-version":[{"id":12065,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/12058\/revisions\/12065"}],"wp:attachment":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/media?parent=12058"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/categories?post=12058"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/tags?post=12058"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}