{"id":11682,"date":"2017-10-14T17:00:55","date_gmt":"2017-10-14T22:00:55","guid":{"rendered":"https:\/\/cancer.wisc.edu\/research\/?p=11682"},"modified":"2019-07-11T17:02:43","modified_gmt":"2019-07-11T22:02:43","slug":"computational-flow-cytometry-analysis-reveals-a-unique-immune-signature-of-the-human-maternal%e2%80%90fetal-interface","status":"publish","type":"post","link":"https:\/\/wwwtest.cancer.wisc.edu\/research\/computational-flow-cytometry-analysis-reveals-a-unique-immune-signature-of-the-human-maternal%e2%80%90fetal-interface\/","title":{"rendered":"Computational flow cytometry analysis reveals a unique immune signature of the human maternal\u2010fetal interface"},"content":{"rendered":"<p><em>This publication used Flow Cytometry in the course of research.<\/em><\/p>\n<div id=\"aji12774-sec-0001\" class=\"article-section__content\">\n<h3 class=\"article-section__sub-title section1\">Problem<\/h3>\n<p>Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual immune interface has hampered the mechanistic investigation.<\/p>\n<\/div>\n<div id=\"aji12774-sec-0002\" class=\"article-section__content\">\n<h3 class=\"article-section__sub-title section1\">Method of study<\/h3>\n<p>Human term decidua was collected, and single\u2010cell phenotypic information was acquired by highly polychromatic flow cytometry. Cellular identity analysis was performed with t\u2010distributed stochastic neighbor embedding, DensVM clustering, and matched to CellOntology database.<\/p>\n<\/div>\n<div id=\"aji12774-sec-0003\" class=\"article-section__content\">\n<h3 class=\"article-section__sub-title section1\">Results<\/h3>\n<p>Traditional analytical methods validated known cellular T and dendritic cell subsets in human term decidua. Computational analysis revealed a complex and tissue\u2010specific decidual immune signature in both the innate and adaptive immune compartments.<\/p>\n<\/div>\n<div id=\"aji12774-sec-0004\" class=\"article-section__content\">\n<h3 class=\"article-section__sub-title section1\">Conclusion<\/h3>\n<p>Polychromatic flow cytometry with a streamlined computational analysis pipeline is a feasible approach to comprehensive immunome mapping of human term decidua. As an unbiased, standardized method of investigation, computational flow cytometry promises to unravel the immune pathology of pregnancy disorders.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual immune interface has hampered the mechanistic investigation.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0},"categories":[131],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/11682"}],"collection":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/comments?post=11682"}],"version-history":[{"count":1,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/11682\/revisions"}],"predecessor-version":[{"id":11683,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/posts\/11682\/revisions\/11683"}],"wp:attachment":[{"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/media?parent=11682"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/categories?post=11682"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wwwtest.cancer.wisc.edu\/research\/wp-json\/wp\/v2\/tags?post=11682"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}